Now, an international team led by RIKEN researchers has mapped the genomic architecture of the odorant receptor repertoire in mice, revealing the start points and promoter regions involved in controlling the expression of the vast majority of these genes. Our study shows that these promoter sequences are strikingly similar between all the olfactory receptors, says Charles Plessy of the RIKEN Omics Science Center in Yokohama, who led the study.
To chart all the promoters, Plessy and colleagues first had to devise an appropriate technique. Plessys collaborator, Piero Carninci also from the RIKEN Omics Science Center, had previously developed a method called cap-analysis gene expression (CAGE) that determines the location of transcription start sites across the whole genome. But CAGE requires large quantities of RNA, which are not always obtainable from particular types of tissues, including the olfactory epithelium. The researchers therefore modified CAGE to nanoCAGE, which requires as little as 10 nanograms of RNA of biological material per sample.
Both CAGE and nanoCAGE work by taking all the messenger RNA transcripts in a given sample, converting them into DNA tags and then comparing the output against a reference genome to find all the transcription start sites. The investigators can then decipher the conserved DNA regulatory sequences in the immediate vicinity of the starting points that form the core promoters.
Using this approach on the minute quantities of RNA found in the olfactory epithelium of mice, Plessys team plotted the promoters of almost 90% of the mouse olfactory receptor genes, as well as the expression of many non-coding regulatory RNAs (Fig. 1). Further bioinformatic analysis of the DNA surrounding the mapped promoters revealed a number of candidate transcription factor binding sites that help control gene expression, some of which the researchers also validated in mouse experiments.
Plessy and colleagues are now adapting the CAGE technique for even smaller biological samples: single cells. Such a method would have wide applications in biology and medicine, and, of course, in the biology of olfactory receptors, says Plessy. With a single-cell technology we will have the potential to make a significant contribution to the field.
More information: Plessy, C., et al. Promoter architecture of mouse olfactory receptor genes. Genome Research advance online publication, 22 December 2011 (doi: 10.1101/gr.126201.111).
Shiraki, T., et al. Cap analysis gene expression for high-throughput analysis of transcriptional starting point and identification of promoter usage. Proceedings of the National Academy of Sciences USA 100, 1577615781 (2003).
Plessy, C., et al. Linking promoters to functional transcripts in small samples with nanoCAGE and CAGEscan. Nature Methods 7, 528534 (2010).
Journal reference:
Genome Research
Proceedings of the National Academy of Sciences
Provided by
RIKEN
<!–
–>
